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Description
Phi X 174 database number # 1CD3. Phi X 174 is a virus that infects bacteria and was the first DNA-based genome to be sequenced in 1977. This bacteriophage has a [+] circular single-stranded DNA genome. The assembly of the capsid has been successfully done in vitro and is the focus of many studies on self-assembly, drug delivery and nanotechnology.
Publication Date
1-18-2013
Keywords
biochemistry, icosahedron, nanobiology, pathogen, teaching, virus
Disciplines
Bacteriology | Biochemistry | Diseases | Immunology and Infectious Disease | Medical Education | Medicine and Health Sciences | Microbiology | Molecular Biology | Virology | Virus Diseases
Creative Commons License
This work is licensed under a Creative Commons Attribution 3.0 License.
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Recommended Citation
Hall, David J., "Phi X 174" (2013). Infectious Pathogens. 3.
https://lux.lawrence.edu/chem_pathogen/3
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Bacteriology Commons, Biochemistry Commons, Immunology and Infectious Disease Commons, Medical Education Commons, Molecular Biology Commons, Virology Commons, Virus Diseases Commons
Instructions
The capsids and nucleocapsids of viruses are only nanometers across and protect the viral genome encased within (http://en.wikipedia.org/wiki/Virus#Structure). The capsids are beautiful examples of icosahedral symmetry and are wonderful examples of self-assembly. Many nanotechnologists are now trying to replicate what nature does so well in order to deliver drugs more effectively among other things. In order to teach about virus structure and self-assembly, I took x-ray crystal structure data and models of various viruses from the VIPER database (.vdb files) (http://viperdb.scripps.edu/) and converted them to .stl files using the program, Chimera (http://www.cgl.ucsf.edu/chimera/). Instructions for converting the model are below as well as a short description of the virus. When slicing the virus, there are some errors; however, the virus prints well. Any suggestions and improvement to the .stl file is appreciated. You can read more about the structures at some of the web links listed at the end.
Phi X 174 database number # 1CD3 Phi X 174 is a virus that infects bacteria and was the first DNA-based genome to be sequenced in 1977. This bacteriophage has a [+] circular single-stranded DNA genome. The assembly of the capsid has been successfully done in vitro and is the focus of many studies on self-assembly, drug delivery and nanotechnology.
Convert vdb to stl file Go to the virus structural database at viperdb.scripps.edu/ Download the virus structure of your choice as a .vdb file Open the .vdb (VIPERdb) file in chimera cgl.ucsf.edu/chimera/ In order to print a virus capsid you shouldn't use the "multiscale" surfaces that appear when you open the VIPERdb file. Make a single surface for the whole virus by creating a simulated density map. Show the command line with Chimera menu Favorites / Command Line... and use molmap #0 10 sym i222 after opening your virus model. The molmap command will make a simulated density map and show a contour surface for that map. The resolution in the example is 10 Angstroms and the protein model #0 is replicated with icosahedral symmetry (with the VIPERdb coordinate system). Switch the volume step size to 1 in the volume dialog (or use command volume #0 step 1) to see the surface at full resolution. (thank you Tom Goddard for suggestions on using chimera) Save as stl file Open in replicator G Scale to appropriate size (many times very small and unseeable!). This can also make the .stl file VERY big. I have no idea why. Upload to cloud.nettfabb.com to fix stl. Sometimes the .stl is unfixable. I do not know why. Slice and print! During slicing, many errors do occur. However, the final model is quite printable and useable. More reading: virology.wisc.edu/virusworld/viruslist.php virology.wisc.edu/virusworld/tri_number.php viperdb.scripps.edu/icos_server.php?icspage=paradigm