Document Type

Honors Project

Publication Date



Increased levels of corticotropin releasing hormone (CRH) are associated with anxiety, as are decreased levels of serotonin (5-HT). Do 5-HT levels influence CRH production and anxiety? The ventral hippocampus is associated with anxiety behavior, contains cells expressing 5-HT receptors (including 5HT7R), and extends axons into CRH producing regions of the hypothalamus (Jalewa et al., 2014; Jiminez et al., 2018). Therefore, 5-HT responsive neurons may play a role in the anxiety response and CRH production. To analyze how 5-HT may influence anxiety, 5dpf Danio rerio were examined following application of a 5-HT7R agonist (AS-19) and antagonist (SB-258179). A distance assay was used to assess physical manifestations of anxiety response. It is hypothesized that separate application of 5-HT7R agonist and antagonist should result in a decrease and increase in the anxiety response, respectively. Furthermore, it is hypothesized CRH levels should increase or decrease in an inverse manner.

Level of Honors

cum laude




Nancy Wall

Included in

Biology Commons